When concentrated nucleic acids are required in downstream applications

Minimize reaction volume, maximize product concentration.

Elute in as little as 8 μL

 

  • We are using this plate on our liquid handler for NGS purposes and works very well in our hands. We were able to lower volumes and thus reduce costs without loss of quality and repetitiveness.

    So an absolute recommendation.

    – Ewart de Bruijn

  • Maximize product concentration.

    Unique magnet architecture for very low volume sample elution.

  • The LE magnet is on its way to being my favorite of the four different types I have to choose from in my lab. I have had no issues using it with single and multichannel pipettes down to as little as 7.5uL elution volumes. Separation times are super fast with low volumes (<20s, generally), and I don’t see any carry over.

    Highly recommended.

    – Peter

How does the LE Magnet Plate work?

The magnets used in the LE Magnet Plate have a smaller hole diameter than the 96R and 96S magnet plates. PCR wells don’t sit as deeply into these magnets, causing the ring of beads to form lower in the well. This means less elution volume is required (approximately 8-10 µl) to fully cover the beads and elute the product.

Full Working Volume

Full Working Volume, Fast Separation Times, Low Elution Volume

Tests with a working solution of Ampure® XP (1 part water + 1.8 parts Ampure) have shown the following settle times:

Alpaqua LE Bead Separation Times

Reaction Volume Separation Time
100 μL 4 minutes
200 μL 6-7 minutes
300 μL < 10 minutes

Important Consideration for Optimum Results

  1. Built For Precision Automated Liquid Handling: The LE Magnet Plate is intended for use with automated liquid handling equipment. Use with manual pipettors is not recommended.  It performs best in conjunction with an advanced liquid handling instrument equipped with a multi-channel dispense head, such as the Beckman Biomek® liquid handlers or the Eppendorf epMotion® platforms.  As a general rule, if your robot can safely work with 384 well PCR plates, the LE Magnet Plate should work well.
  2. Only Compatible With Flat PCR Plates: It is very important to only use flat PCR plates with the LE Magnet Plate. All wells must touch the LE magnets so the ring of magnetic beads will form at the correct position in the wells. Do not use warped PCR plates because if the wells do not touch the LE magnets, the magnetic beads will cluster at the bottom and cause bead carry-over in those wells.  The LE is not for use with single PCR strips.

Recommended PCR Microplates

The LE Magnet Plate works with most 96-well PCR plates – full-, semi-, or non-skirted.  The LE is not for use with single PCR strips.  Below are some common plates that we have confirmed reliably work.   Many others will work.  Please Contact Alpaqua if you would like to inquire about the compatibility of your specific PCR plate.

  • Eppendorf twin.tec 96 (full, semi, and non-skirted versions)
  • Abgene SuperPlate
  • Abgene AB-0800,  AB-1000 & Axygen PCR-96-FS-C
  • BioRad Hard-Shell Plate

 

Not Recommended: ABI MicroAmp® Fast 96-Well Reaction Plate, 0.1 mL

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