Minimize reaction volume, maximize product concentration.
Elute in as little as 8 μL
We are using this plate on our liquid handler for NGS purposes and works very well in our hands. We were able to lower volumes and thus reduce costs without loss of quality and repetitiveness.
So an absolute recommendation.
– Ewart de Bruijn
Unique magnet architecture for very low volume sample elution.
The LE magnet is on its way to being my favorite of the four different types I have to choose from in my lab. I have had no issues using it with single and multichannel pipettes down to as little as 7.5uL elution volumes. Separation times are super fast with low volumes (<20s, generally), and I don’t see any carry over.
The magnets used in the LE Magnet Plate have a smaller hole diameter than the 96R and 96S magnet plates. PCR wells don’t sit as deeply into these magnets, causing the ring of beads to form lower in the well. This means less elution volume is required (approximately 8-10 µl) to fully cover the beads and elute the product.
Full Working Volume, Fast Separation Times, Low Elution Volume
Tests with a working solution of Ampure® XP (1 part water + 1.8 parts Ampure) have shown the following settle times:
|Reaction Volume||Separation Time|
|100 μL||4 minutes|
|200 μL||6-7 minutes|
|300 μL||< 10 minutes|
The LE Magnet Plate works with most 96-well PCR plates – full-, semi-, or non-skirted. The LE is not for use with single PCR strips. Below are some common plates that we have confirmed reliably work. Many others will work. Please Contact Alpaqua if you would like to inquire about the compatibility of your specific PCR plate.
Not Recommended: ABI MicroAmp® Fast 96-Well Reaction Plate, 0.1 mL